Convince your local public library to offer self-serve kiosks so patrons can check their materials in and out.
- Write a proposal of not more than 650 words that evaluates the operational, technical, and economic feasibility of these kiosks.
- Draw use-case diagrams that illustrate how a patron would interact with the kiosk. These diagrams should illustrate all of the interactions in enough detail to derive functional requirements for the kiosk.
Assume that all materials and library cards carry a unique bar code.
The patron should have the option to receive a receipt for items checked in or out.
- Write sample instructions on how to use the kiosk. The library intends to post these instructions on a sign next to the kiosk, so they must be short (not more than 350 words), clear, complete, and well organized.
Sample Solution
ell as T3 RNA polymerase promoters which are used towards in vitro RNA synthesis. The promoterâs choice serves towards initiating transcription which indeed determines the strand to be cloned by insertion into the polylinker that will be indeed transcribed. The transcripts were isolated from both the tyrosine kinase inhibitor treated as well as the untreated KCL22 cells. For successful cloning, RNA was extracted subsequently cDNA was subjected to amplification using PCR. The plasmids were purified, digested and the amplified cDNA was cloned and transformed into vector. Human leukemic cell line (KCL22) were selected for this study. The Kcl22 cell line is being used extensively owing to its use as exceedingly sensitive target in vitro for various assays. Cultures of ATCC stock exhibit sensitivity towards assessment of human natural killer activity. They are Chronic myelogenous leukemia, BCR-ABL1 positive cell line.These are hematopoietic stem cells having capacity to differentiate to form erythroid, megakaryocytic lineages. The entire RNA content was extracted from KCL22 cell lines, and it was subjected to reverse transcription. The obtained CDNA quality was affirmed by real time qPCR. Amplified product of TROVE2 transcript was cloned into vector subsequent to digestion with selected restriction digestion with pst1 and sgf1. Then, it was ligated by using Bioline QS ligase enzyme. Transformation was carried by heat shock method. Blue white selection strategy was employed to select the transformed colonies. Colony PCR was performed for ascertaining the proper cloning. Agarose gel electrophoresis was employed to separate DNA fragments and analysed fragments cross checked for ascertainment. Results: Tissue culture of KCL22 cells were grown as per routine standard tissue culture conditions. Treated KCL22 cellular samples were grown with media being supplemented with tyrosine kinase inhibitor( TKI) drug for a period of 72h whereas untreated cells were indeed cultured in medium devoid of TKI drug. The morphology of KCL22 cells were subsequently observed by inverted microscope. Via>
ell as T3 RNA polymerase promoters which are used towards in vitro RNA synthesis. The promoterâs choice serves towards initiating transcription which indeed determines the strand to be cloned by insertion into the polylinker that will be indeed transcribed. The transcripts were isolated from both the tyrosine kinase inhibitor treated as well as the untreated KCL22 cells. For successful cloning, RNA was extracted subsequently cDNA was subjected to amplification using PCR. The plasmids were purified, digested and the amplified cDNA was cloned and transformed into vector. Human leukemic cell line (KCL22) were selected for this study. The Kcl22 cell line is being used extensively owing to its use as exceedingly sensitive target in vitro for various assays. Cultures of ATCC stock exhibit sensitivity towards assessment of human natural killer activity. They are Chronic myelogenous leukemia, BCR-ABL1 positive cell line.These are hematopoietic stem cells having capacity to differentiate to form erythroid, megakaryocytic lineages. The entire RNA content was extracted from KCL22 cell lines, and it was subjected to reverse transcription. The obtained CDNA quality was affirmed by real time qPCR. Amplified product of TROVE2 transcript was cloned into vector subsequent to digestion with selected restriction digestion with pst1 and sgf1. Then, it was ligated by using Bioline QS ligase enzyme. Transformation was carried by heat shock method. Blue white selection strategy was employed to select the transformed colonies. Colony PCR was performed for ascertaining the proper cloning. Agarose gel electrophoresis was employed to separate DNA fragments and analysed fragments cross checked for ascertainment. Results: Tissue culture of KCL22 cells were grown as per routine standard tissue culture conditions. Treated KCL22 cellular samples were grown with media being supplemented with tyrosine kinase inhibitor( TKI) drug for a period of 72h whereas untreated cells were indeed cultured in medium devoid of TKI drug. The morphology of KCL22 cells were subsequently observed by inverted microscope. Via>
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