After incubating the plates, the resulting bacterial growth on each of the four plates were observed and the morphologies and colors were recorded. One yellow colony and one off-white colony were taken from the 37 degrees Celsius TSA plate, and were streaked for isolation on two separate TSA plates, which were then incubated for 1-2 days. After this incubation period, Gram stains were performed from the two new subcultures to ensure that the two bacteria had been isolated and to distinguish between the Gram positive unknown and the Gram negative unknown. The size, shape, color, and arrangement for both types of bacteria were recorded before moving on with the experiment. The first test carried out for the Gram positive bacteria was a catalase test, checking for the presence of the enzyme catalase. To perform this test, a sterilized inoculating loop was used to streak a sample of the bacteria onto a glass slide, and a drop of hydrogen peroxide was added to the bacteria. The production of gas bubbles would indicate the presence of catalase, because this would mean that the bacterium was able to convert hydrogen peroxide into water and oxygen. The results of the catalase test were recorded. After conducting this test, a mannitol fermentation test was carried out. This was done by creating an isolation streak on an MSA plate and incubating the plate at 37 degrees Celsius for 1-2 days. A yellow halo around the bacteria on the MSA plate would indicate that they fermented mannitol, while no change in color in the MSA plate would mean that the bacteria did not ferment mannitol. Observations were recorded for this test. After confirming the results by observing the colony morphology under a microscope and the color on a TSA plate, no other tests were needed to determine the Gram positive unknown bacteria according to the dichotomous key. The process of identifying the Gram negative bacteria was not as simple, because there>