Quantitate anti-D levels in plasma using flow cytometry and haemagglutination

please follow the steps and summarize as possible. not more than 3 lines in each.
1-why used R2R2 red cells for quantitate anti-D by flow cytometry.
2-how PBS work as removed antibody ( mechanism) for quantitate anti-D?
3- differentiate between whole molecules IgG antibody and Fab IgG antibody and affect them on flow cytometry technique.
4- as we know Fab IgG antibody should be store at 4 degrees on the fridge . what effect room temperature on mechanism of Fab IgG ?
5- effect room temperature and incubation 37 degrees on methods anti-D using flow cytometry.
6- talk about kleihaure-betke acid elution technique. Please add figure.
7-talk about population differences Rh D positive, Rh D negative, immunogenicity of D antigen, HTR against HDFN and kleihaure-betke. Please use diagram to explain mechanism of HDFN.
8- principle of flow cytometry BD FACS canto II.

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