Guidance for Fluorescence Lab Report

1. Water: Described the spectra to the reader in terms of the types of scattering that can be observed.
2. Spectra of Phenanthrene (without quencher): Described the emission and excitation spectra, note the wavelengths of highest signal intensity. Determine the Stokes shift.Compare the Excitation spectrum with the UVvis absorption spectrum (posted on BlackBoard in Results folder). Please note that the relative intensities of signals in UVvis spectrum may differ from the excitation spectrum collected with a Fluorescence spectrometer.
3. Quenching experiments: Create the Stern-Volmer plots for the quenching experiment with phenanthrene; one for quenching with 1,10-phenanthroline and one for chloride. Note that you have to calculate the concentration of the quencher (1,10-phenanthroline) in the solution present in the cuvet after each addition. You can assume that theinitial volume was 3 mL. Using the general dilution equation C1 × V1 = C2 × V2 , the quencher concentration can be calculated after each addition (see also example below). The 1,10-phenanthroline stock solution was 0.005 M; the sodium chloride solution was 0.10 M.
4. Determine the quenching constant for each quencher based on the Stern-Volmer plot.
5. Discuss the quality of the data based on the Stern-Volmer plots.
6. Make sure that the discussion includes a comparison ofthe quenching abilities of 1,10-phenanthroline and chloride. Include the terms static quenching and dynamic quenching in your discussion.