Flow Cytometry assessment of pancreatic islets, as a predictor of transplant outcome

Please follow these steps
1. Topic of Study (Flow Cytometry assessment of pancreatic islets, as a predictor of transplant outcome)
2. Introduction
3. Significance of the Study
4. Literature Review
5. Methodology.
6. Expected Results.
7. Ethical Considerations.
8. Time-Frame and Budget
9. References

You may use this
Krull, D. (2011). Quantitative Assessment of Pancreatic Islets Using Laser Scanning Cytometry.Current Protocols in Cytometry. doi:10.1002/0471142956.cy0632s56
Todorov, I., Nair, I., Avakian-Mansoorian, A., Rawson, J., Omori, K., Ito, T., � Kandeel, F. (2010). Quantitative Assessment of ?-Cell Apoptosis and Cell Composition of Isolated, Undisrupted Human Islets by Laser Scanning Cytometry. Transplantation, 90(8), 836-842. doi:10.1097/tp.0b013e3181f1db5d
Ichii, H., Inverardi, L., Pileggi, A., Molano, R. D., Cabrera, O., Caicedo, A., � Ricordi, C. (2005). A Novel Method for the Assessment of Cellular Composition and Beta-Cell Viability in Human Islet Preparations. American Journal of Transplantation, 5(7), 1635-1645. doi:10.1111/j.1600-6143.2005.00913.x
Misawa, R., Miki, A., Barker, S., Mita, A., Ricordi, C., Kahn, A., � Ichii, H. (2010). SERVA COLLAGENASE NB1: COMPARISON WITH LIBERASE HI AND INTERLOT VARIABILITY. Transplantation Journal, 90, 1016. doi:10.1097/00007890-201007272-01993
Misawa, R., Ricordi, C., Miki, A., Barker, S., Molano, R. D., Khan, A., � Ichii, H. (2012). Evaluation of Viable ?-Cell Mass Is Useful for Selecting Collagenase for Human Islet Isolation: Comparison of Collagenase NB1 and Liberase HI. Cell Transplantation,21(1), 39-47. doi:10.3727/096368911×582732
Mita, A., Ricordi, C., Messinger, S., Miki, A., Misawa, R., Barker, S., � Ichii, H. (2010). Antiproinflammatory Effects of Iodixanol (OptiPrep)-Based Density Gradient Purification on Human Islet Preparations. Cell Transplantation, 19(12), 1537-1546. doi:10.3727/096368910×516600
Buchwald, P., Wang, X., Khan, A., Bernal, A., Fraker, C., Inverardi, L., & Ricordi, C. (2009). Quantitative Assessment of Islet Cell Products: Estimating the Accuracy of the Existing Protocol and Accounting for Islet Size Distribution. Cell Transplantation, 18(10), 1223-1235. doi:10.3727/096368909×476968
Papas, K. K., Suszynski, T. M., & Colton, C. K. (2009). Islet assessment for transplantation. Current Opinion in Organ Transplantation, 14(6), 674-682. doi:10.1097/mot.0b013e328332a489