CELL VIABILITY and how to measure it.
Answer the following questions:
1. MTT assay description; what it is used for, what happens in the cell, how is it viability measured.See Roche web site for help. What are advantages and limitations of MTT assay.
2. Take paper 1 with focus on your cell line, and dietary agent, and MTT result. Copy the table or the graph, explain what happened in the study. What cancer did you study? Use terms dose dependence or concdependence , or time dependent study if you see it.
3. Any out of the ordinary result is always exciting, so that can be explained. Show the graphs, discuss results.
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